Two-s i t e assay of human o1-1etoprotein using 1251-labelled monoclonal ant ibodies
نویسندگان
چکیده
Monoclonal antibodies to human alietoprotein (AFP) have been compared with a conventionally produced a n t i s e r u m us ing r a d i o i m m u n o a s s a y and twos i t e immunoradiometric techniques. A low-affinity antibody, which proved inadequate for use in a radioimmunoassay, gave a s a t i s f a c t o r y dose-response Curve in a rapid two-site assay. A higher-affinity antibody yielded a simple, rapid, and sensitive two-site assay suitable for routine measurement of serum AFP.
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